Hotspot

rpl36-infA

LSC · 79075-79169 · 95 bp

Back to Ruellia

Pi 0.0211

MarkerSeek score 64.60

Primer available yes

Label rpl36-infA

Feature Metadata

label_name: rpl36-infA
feature_type: igs
region: LSC
length_bp: 95
variable_sites: 2
pi: 0.0211
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-infA_p1	ATTATTCTAATGCGCCCCCG	ATACTGCCGGGGGATAAAGT	50.0	50.0	58.2	58.2	271 bp	1.0	77.7
rpl36-infA_p2	CCTGCGGATTAGTCGACATT	ATACTGCCGGGGGATAAAGT	50.0	50.0	57.8	58.2	251 bp	1.0	77.4
rpl36-infA_p3	ATTATTCTAATGCGCCCCCG	ACTGCCGGGGGATAAAGTAA	50.0	50.0	58.2	58.4	269 bp	1.0	75.9
rpl36-infA_p4	CCTGCGGATTAGTCGACATT	ACTGCCGGGGGATAAAGTAA	50.0	50.0	57.8	58.4	249 bp	1.0	75.5
rpl36-infA_p5	ATTATTCTAATGCGCCCCCG	GATACTGCCGGGGGATAAAG	50.0	55.0	58.2	57.5	272 bp	1.0	75.4