Hotspot

rpl20-rps12

LSC · 68498-69277 · 780 bp

Back to Ruellia

Pi 0.0115

MarkerSeek score 56.50

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 780
variable_sites: 9
pi: 0.0115
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TCCGAGCTATATATCCGCGT	ACAGCCGATTCGAAATGTCA	50.0	45.0	58.0	57.9	905 bp	1.0	79.8
rpl20-rps12_p2	TCCGAGCTATATATCCGCGT	AGCCGATTCGAAATGTCACA	50.0	45.0	58.0	57.9	903 bp	1.0	79.8
rpl20-rps12_p3	TCCGAGCTATATATCCGCGT	ACTAGGGTGTATGTGCGACT	50.0	50.0	58.0	58.2	838 bp	1.0	79.4
rpl20-rps12_p4	TCCGAGCTATATATCCGCGT	ACAAGACAGCCGATTCGAAA	50.0	45.0	58.0	57.8	910 bp	1.0	79.2
rpl20-rps12_p5	TCCGAGCTATATATCCGCGT	AGACAGCCGATTCGAAATGT	50.0	45.0	58.0	57.6	907 bp	1.0	77.4