Hotspot

rpl33-rps18

LSC · 67400-67578 · 179 bp

Back to Ruellia

Pi 0.0223

MarkerSeek score 65.30

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 179
variable_sites: 4
pi: 0.0223
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	ACTCAAAAGAACCGGCACAA	CAATTCGATCCCCCGATTGA	45.0	50.0	58.2	57.7	363 bp	1.0	76.9
rpl33-rps18_p2	ACTCAAAAGAACCGGCACAA	TCAATTCGATCCCCCGATTG	45.0	50.0	58.2	57.7	364 bp	1.0	76.9
rpl33-rps18_p3	ACTCAAAAGAACCGGCACAA	TTCGATCCCCCGATTGAATC	45.0	50.0	58.2	57.5	360 bp	1.0	74.6
rpl33-rps18_p4	TACTCAAAAGAACCGGCACA	CAATTCGATCCCCCGATTGA	45.0	50.0	57.4	57.7	364 bp	1.0	73.9
rpl33-rps18_p5	TACTCAAAAGAACCGGCACA	TCAATTCGATCCCCCGATTG	45.0	50.0	57.4	57.7	365 bp	1.0	73.8