Hotspot

psaA-ycf3

LSC · 41889-42584 · 696 bp

Back to Ruellia

Pi 0.0115

MarkerSeek score 55.80

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 696
variable_sites: 8
pi: 0.0115
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TTTTACTTCTGGTTCCGGCG	ATTGCAGAGGCTTGGTTTGA	50.0	45.0	58.5	58.0	846 bp	1.0	76.9
psaA-ycf3_p2	TTTTACTTCTGGTTCCGGCG	TTGCAGAGGCTTGGTTTGAT	50.0	45.0	58.5	58.0	845 bp	1.0	76.9
psaA-ycf3_p3	TTTTACTTCTGGTTCCGGCG	AGCCCTTACCCCTGGTAATT	50.0	50.0	58.5	58.0	796 bp	1.0	76.9
psaA-ycf3_p4	TTTTACTTCTGGTTCCGGCG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.5	58.0	827 bp	1.0	76.6
psaA-ycf3_p5	TTTTACTTCTGGTTCCGGCG	TTGAAGATCACGAGGCGTTT	50.0	45.0	58.5	57.8	756 bp	1.0	75.7