Hotspot

trnS-GCU-trnS-CGA

LSC · 8191-8961 · 771 bp

Back to Ruellia

Pi 0.0104

MarkerSeek score 57.20

Primer available yes

Label trnS-GCU-trnS-CGA

Feature Metadata

label_name: trnS-GCU-trnS-CGA
feature_type: igs
region: LSC
length_bp: 771
variable_sites: 8
pi: 0.0104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnS-CGA_p1	CAATCCGACGCTTTAGTCCA	ACCACTAAACTACACCCGCT	50.0	50.0	58.0	58.7	826-836 bp	1.0	75.6
trnS-GCU-trnS-CGA_p2	AATCCGACGCTTTAGTCCAC	ACCACTAAACTACACCCGCT	50.0	50.0	58.0	58.7	825-835 bp	1.0	75.6
trnS-GCU-trnS-CGA_p3	ACGGAAAGAGAGGGATTCGA	ACCACTAAACTACACCCGCT	50.0	50.0	58.2	58.7	880-890 bp	1.0	74.4
trnS-GCU-trnS-CGA_p4	AGCAATCCGACGCTTTAGTC	ACCACTAAACTACACCCGCT	50.0	50.0	58.4	58.7	828-838 bp	1.0	72.8
trnS-GCU-trnS-CGA_p5	CGACGCTTTAGTCCACTCAG	ACCACTAAACTACACCCGCT	55.0	50.0	58.4	58.7	821-831 bp	1.0	72.6