Hotspot

trnH-GUG-psbA

LSC · 105-509 · 405 bp

Back to Ruellia

Pi 0.015
MarkerSeek score 56.00
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
405
variable_sites
6
pi
0.015
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG CGTGCTAACCTTGGTATGGA 55.0 50.0 58.1 57.3 519-521 bp 1.0 75.3
trnH-GUG-psbA_p2 GATCCACTTGGCTACATCCG TCGTGCTAACCTTGGTATGG 55.0 50.0 58.1 57.3 520-522 bp 1.0 75.3
trnH-GUG-psbA_p3 CAATCCACTGCCTTGATCCA CGTGCTAACCTTGGTATGGA 50.0 50.0 57.9 57.3 533-535 bp 1.0 75.3
trnH-GUG-psbA_p4 CAATCCACTGCCTTGATCCA TCGTGCTAACCTTGGTATGG 50.0 50.0 57.9 57.3 534-536 bp 1.0 75.3
trnH-GUG-psbA_p5 AATCCACTGCCTTGATCCAC CGTGCTAACCTTGGTATGGA 50.0 50.0 57.9 57.3 532-534 bp 1.0 75.3