Hotspot

rpl16

LSC · 82799-84154 · 1356 bp

Back to Rubus

Pi 0.0185
MarkerSeek score 65.60
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1356
variable_sites
204
pi
0.0185
unique_haplotype_count
68
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 TGTCTTAATTCCCGGGGGAT TCCAAACCATTCGAGCCAAA 50.0 45.0 58.1 58.0 1999-2065 bp 1.0 91.1
rpl16_p2 TGTCTTAATTCCCGGGGGAT CAAGTACAAATTGCAGGGCG 50.0 50.0 58.1 58.0 2075-2141 bp 1.0 91.1
rpl16_p3 CCGCTGTTATCCGCTACATT TCCAAACCATTCGAGCCAAA 50.0 45.0 58.1 58.0 1720-1786 bp 1.0 90.8
rpl16_p4 ACAATTACAGCTCGGACCAC TCCAAACCATTCGAGCCAAA 50.0 45.0 57.9 58.0 1878-1900 bp 0.4 57.8
rpl16_p5 ACAATTACAGCTCGGACCAC CAAGTACAAATTGCAGGGCG 50.0 50.0 57.9 58.0 1954-1976 bp 0.4 57.6