Hotspot

rpl20-clpP1

LSC · 70346-71417 · 1072 bp

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Pi 0.0166
MarkerSeek score 67.20
Primer available yes
Label rpl20-clpP1

Feature Metadata

label_name
rpl20-clpP1
feature_type
igs
region
LSC
length_bp
1072
variable_sites
152
pi
0.0166
unique_haplotype_count
67
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-clpP1_p1 TTGTTCTACGTCTTCGAGCT TATGTCAGCAACAGAAGCCC 45.0 50.0 56.6 57.9 1156-1208 bp 0.988235 79.7
rpl20-clpP1_p2 TTGTTCTACGTCTTCGAGCT TATGCACAAAGGACAGGCAA 45.0 45.0 56.6 57.7 1218-1270 bp 0.988235 78.3
rpl20-clpP1_p3 TTTGTTCTACGTCTTCGAGCT TATGTCAGCAACAGAAGCCC 42.9 50.0 57.4 57.9 1157-1209 bp 0.988235 77.5
rpl20-clpP1_p4 TTGTTCTACGTCTTCGAGCT AGGACAGGCAAACCCTTATG 45.0 50.0 56.6 57.5 1209-1261 bp 0.988235 76.5
rpl20-clpP1_p5 TTGTTCTACGTCTTCGAGCT GGACAGGCAAACCCTTATGG 45.0 55.0 56.6 58.5 1208-1260 bp 0.988235 76.4