Hotspot

psbE-petL

LSC · 65990-67203 · 1214 bp

Back to Rubus

Pi 0.0214
MarkerSeek score 75.40
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1214
variable_sites
207
pi
0.0214
unique_haplotype_count
66
primer_available
yes
alignment_reliability
0.99
markerseek_score
75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 AGAACGTTCTCCTGTGCTTC AAGGCGTATCTTGCTCAGAC 50.0 50.0 57.8 57.7 1115-1346 bp 1.0 88.3
psbE-petL_p2 ACGAATAACCAACCCGCAAT AAGGCGTATCTTGCTCAGAC 45.0 50.0 58.2 57.7 1201-1432 bp 1.0 88.2
psbE-petL_p3 TGCTGACGAATAACCAACCC AAGGCGTATCTTGCTCAGAC 50.0 50.0 58.2 57.7 1206-1437 bp 1.0 88.1
psbE-petL_p4 GTTCTCCTGTGCTTCCAGAC AAGGCGTATCTTGCTCAGAC 55.0 50.0 58.2 57.7 1110-1341 bp 1.0 88.0
psbE-petL_p5 TCTCCTGTGCTTCCAGACAT AAGGCGTATCTTGCTCAGAC 50.0 50.0 58.4 57.7 1108-1339 bp 1.0 86.8