Hotspot

ndhC-trnV-UAC

LSC · 52412-53037 · 626 bp

Back to Rubus

Pi 0.0364
MarkerSeek score 81.40
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
626
variable_sites
193
pi
0.0364
unique_haplotype_count
66
primer_available
yes
alignment_reliability
0.95
markerseek_score
81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGGGCTAAAACTCCGGAAAT GCACGTTGGGTCTTTGAAAC 50.0 50.0 57.9 58.2 543-862 bp 1.0 89.6
ndhC-trnV-UAC_p2 GGGGCTAAAACTCCGGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 479-798 bp 1.0 89.3
ndhC-trnV-UAC_p3 CTGGTCCTTTGCTAATCGGG GCACGTTGGGTCTTTGAAAC 55.0 50.0 58.3 58.2 560-879 bp 1.0 88.0
ndhC-trnV-UAC_p4 CCTTTGCTAATCGGGGCTAA GCACGTTGGGTCTTTGAAAC 50.0 50.0 57.7 58.2 555-874 bp 1.0 87.9
ndhC-trnV-UAC_p5 CTGGTCCTTTGCTAATCGGG AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.3 57.8 496-815 bp 1.0 87.8