Hotspot

trnF-GAA-ndhJ

LSC · 49987-50722 · 736 bp

Back to Rubus

Pi 0.0227
MarkerSeek score 67.20
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
736
variable_sites
155
pi
0.0227
unique_haplotype_count
62
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 GGGATAGCTCAGCTGGTAGA TGAAATACAAGATGCTCATTGA 55.0 31.8 58.0 53.8 540-882 bp 1.0 52.0
trnF-GAA-ndhJ_p2 CCTCGTGTCACCAGTTCAAA TGAAATACAAGATGCTCATTGA 50.0 31.8 58.1 53.8 504-846 bp 1.0 52.0
trnF-GAA-ndhJ_p3 GTCGTGAATGGTCGGGATAG TGAAATACAAGATGCTCATTGA 55.0 31.8 57.9 53.8 553-895 bp 1.0 52.0
trnF-GAA-ndhJ_p4 CTGGTAGAGCAGAGGACTGA TGAAATACAAGATGCTCATTGA 55.0 31.8 57.9 53.8 528-870 bp 1.0 52.0
trnF-GAA-ndhJ_p5 GTGAATGGTCGGGATAGCTC TGAAATACAAGATGCTCATTGA 55.0 31.8 57.8 53.8 550-892 bp 1.0 52.0