Hotspot

trnT-GGU-psbD

LSC · 32210-33568 · 1359 bp

Back to Rubus

Pi 0.0241
MarkerSeek score 73.00
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1359
variable_sites
280
pi
0.0241
unique_haplotype_count
71
primer_available
yes
alignment_reliability
0.99
markerseek_score
73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG ACAAAAACGAAACGGTCCCT 55.0 45.0 58.1 58.2 1408-1662 bp 1.0 89.5
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA ACAAAAACGAAACGGTCCCT 50.0 45.0 58.1 58.2 1411-1665 bp 1.0 89.3
trnT-GGU-psbD_p3 GTGGTAGAGTAACGCCATGG CAAAAACGAAACGGTCCCTC 55.0 50.0 58.1 57.6 1407-1661 bp 0.988235 87.9
trnT-GGU-psbD_p4 GGCGTAAGTCATCGGTTCAA ACAAAAACGAAACGGTCCCT 50.0 45.0 58.3 58.2 1385-1639 bp 1.0 87.8
trnT-GGU-psbD_p5 TCAGTGGTAGAGTAACGCCA CAAAAACGAAACGGTCCCTC 50.0 50.0 58.1 57.6 1410-1664 bp 0.988235 87.7