Hotspot

rpoB-trnC-GCA

LSC · 26630-27577 · 948 bp

Back to Rubus

Pi 0.0246
MarkerSeek score 82.30
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
948
variable_sites
182
pi
0.0246
unique_haplotype_count
71
primer_available
yes
alignment_reliability
0.99
markerseek_score
82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 TATTGCCTCATTTCCACCCC CCGGATTTGAACTGGGGAAA 50.0 50.0 57.5 58.1 869-1046 bp 0.988235 87.3
rpoB-trnC-GCA_p2 TATTGCCTCATTTCCACCCC AAAAGGATTTGCAGTCCCCC 50.0 50.0 57.5 58.4 851-1028 bp 0.988235 85.1
rpoB-trnC-GCA_p3 ATATTGCCTCATTTCCACCCC CCGGATTTGAACTGGGGAAA 47.6 50.0 58.0 58.1 870-1047 bp 0.988235 82.6
rpoB-trnC-GCA_p4 CATTTCCACCCCCAAGCAT CCGGATTTGAACTGGGGAAA 52.6 50.0 58.0 58.1 861-1038 bp 0.964706 81.9
rpoB-trnC-GCA_p5 GTTCCTCTGTTAAGCCCTGA CCGGATTTGAACTGGGGAAA 50.0 50.0 56.8 58.1 945-1122 bp 0.988235 81.7