Hotspot

trnS-GCU-trnG-GCC

LSC · 8499-9096 · 598 bp

Back to Rubus

Pi 0.0286
MarkerSeek score 71.30
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
598
variable_sites
140
pi
0.0286
unique_haplotype_count
58
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 CAATCCGACGCTTTAGTCCA GGGGGTTATAGAACGAATCACA 50.0 45.5 58.0 57.6 637-707 bp 0.964706 71.3
trnS-GCU-trnG-GCC_p2 AATCCGACGCTTTAGTCCAC GGGGGTTATAGAACGAATCACA 50.0 45.5 58.0 57.6 636-706 bp 0.964706 71.3
trnS-GCU-trnG-GCC_p3 ACGGAAAGAGAGGGATTCGA GGGGGTTATAGAACGAATCACA 50.0 45.5 58.2 57.6 691-761 bp 0.964706 70.1
trnS-GCU-trnG-GCC_p4 CAATCCGACGCTTTAGTCCA AGGGGGTTATAGAACGAATCAC 50.0 45.5 58.0 57.3 638-708 bp 0.964706 69.1
trnS-GCU-trnG-GCC_p5 AATCCGACGCTTTAGTCCAC AGGGGGTTATAGAACGAATCAC 50.0 45.5 58.0 57.3 637-707 bp 0.964706 69.1