Hotspot

trnK-UUU-rps16

LSC · 4239-5267 · 1029 bp

Back to Rubus

Pi 0.0361
MarkerSeek score 74.60
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
1029
variable_sites
296
pi
0.0361
unique_haplotype_count
76
primer_available
yes
alignment_reliability
0.98
markerseek_score
74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCTTTCAGGATCAGTCGTGG CGACGAGAGGGAAGAGATCT 55.0 55.0 58.0 58.0 1059-1381 bp 1.0 91.6
trnK-UUU-rps16_p2 CCTTTCAGGATCAGTCGTGG AAAAAGGCGCTCAACCTACA 55.0 45.0 58.0 58.0 965-1287 bp 0.988235 91.2
trnK-UUU-rps16_p3 TCAGGATCAGTCGTGGTCTT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.1 58.0 961-1283 bp 0.988235 90.7
trnK-UUU-rps16_p4 TTCAGGATCAGTCGTGGTCT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.1 58.0 962-1284 bp 0.988235 90.7
trnK-UUU-rps16_p5 CCTTTCAGGATCAGTCGTGG TGTTGCTGCCGTTTTGAAAG 55.0 45.0 58.0 58.1 1555-1896 bp 0.964706 89.8