Hotspot

ycf2-trnI-CAU

IRa · 149141-149202 · 62 bp

Back to Rubroshorea

Pi 0.179

MarkerSeek score 85.90

Primer available yes

Label ycf2-trnI-CAU

Feature Metadata

label_name: ycf2-trnI-CAU
feature_type: igs
region: IRa
length_bp: 62
variable_sites: 10
pi: 0.179
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 85.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnI-CAU_p1	TGAATTGACTTGGGTCCACG	GTTGGGCGCTTTAACCATTC	50.0	50.0	58.1	58.0	196-216 bp	1.0	85.3
ycf2-trnI-CAU_p2	GAATTGACTTGGGTCCACGA	GTTGGGCGCTTTAACCATTC	50.0	50.0	57.8	58.0	195-215 bp	1.0	84.7
ycf2-trnI-CAU_p3	AATTGACTTGGGTCCACGAG	GTTGGGCGCTTTAACCATTC	50.0	50.0	57.8	58.0	194-214 bp	1.0	84.7
ycf2-trnI-CAU_p4	TTGACTTGGGTCCACGAGTA	GTTGGGCGCTTTAACCATTC	50.0	50.0	58.3	58.0	192-212 bp	1.0	83.9
ycf2-trnI-CAU_p5	CCCACTGAATTGACTTGGGT	GTTGGGCGCTTTAACCATTC	50.0	50.0	57.7	58.0	201-221 bp	1.0	83.7