Hotspot

rpl32-ndhF

SSC · 124501-125401 · 901 bp

Back to Rubroshorea

Pi 0.0415

MarkerSeek score 70.50

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 901
variable_sites: 37
pi: 0.0415
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	AAAAAGAATCCCCATCCCGG	AGGGGAAATTGTGCGGATTT	50.0	45.0	57.8	58.1	1888-2010 bp	1.0	84.2
rpl32-ndhF_p2	AAAAAGAATCCCCATCCCGG	TGTTGCTGTCGGAAAAAGGA	50.0	45.0	57.8	58.0	1372 bp	0.5	58.4
rpl32-ndhF_p3	CCCCATCCCGGAAAGAAAAT	TGTTGCTGTCGGAAAAAGGA	50.0	45.0	57.8	58.0	1363 bp	0.5	58.4
rpl32-ndhF_p4	CCCCATCCCGGAAAGAAAAT	AAGGGGAAATTGTGCGGATT	50.0	45.0	57.8	58.1	2002 bp	0.5	58.2
rpl32-ndhF_p5	CCCCATCCCGGAAAGAAAAT	AGGGGAAATTGTGCGGATTT	50.0	45.0	57.8	58.1	2001 bp	0.5	58.2