Hotspot

trnL-UAG-rpl32

SSC · 123005-124326 · 1322 bp

Back to Rubroshorea

Pi 0.0351

MarkerSeek score 66.80

Primer available yes

Label trnL-UAG-rpl32

Feature Metadata

label_name: trnL-UAG-rpl32
feature_type: igs
region: SSC
length_bp: 1322
variable_sites: 41
pi: 0.0351
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-rpl32_p1	AAAAGGCCCGAAGTAGCAAA	AAGTGGACTGCGACAACAAT	45.0	45.0	58.0	58.0	2227-2353 bp	1.0	86.0
trnL-UAG-rpl32_p2	GCTGGTACTAATGTTGCGGA	AAGTGGACTGCGACAACAAT	50.0	45.0	58.0	58.0	2263-2389 bp	1.0	85.9
trnL-UAG-rpl32_p3	AGCTGGTACTAATGTTGCGG	AAGTGGACTGCGACAACAAT	50.0	45.0	58.0	58.0	2264-2390 bp	1.0	85.9
trnL-UAG-rpl32_p4	ACGACTGAAACGACCGAAAA	AAGTGGACTGCGACAACAAT	45.0	45.0	58.1	58.0	1936-2062 bp	1.0	85.7
trnL-UAG-rpl32_p5	TTGTTCCCTTTGCGGATGAA	AAGTGGACTGCGACAACAAT	45.0	45.0	58.0	58.0	2136 bp	0.5	59.6