Hotspot

trnN-GUU-ycf1

IRb · 107329-107767 · 439 bp

Back to Rubroshorea

Pi 0.052

MarkerSeek score 74.30

Primer available yes

Label trnN-GUU-ycf1

Feature Metadata

label_name: trnN-GUU-ycf1
feature_type: igs
region: IRb
length_bp: 439
variable_sites: 22
pi: 0.052
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ycf1_p1	AGGATTCGAACCTACGACCA	ATTACCTGGTATAAACGGCA	50.0	40.0	58.2	54.1	521-522 bp	1.0	53.6
trnN-GUU-ycf1_p2	AGGATTCGAACCTACGACCA	TTACCTGGTATAAACGGCAT	50.0	40.0	58.2	54.1	520-521 bp	1.0	53.6
trnN-GUU-ycf1_p3	CCCCAAGTAGGATTCGAACC	ATTACCTGGTATAAACGGCA	55.0	40.0	57.7	54.1	529-530 bp	1.0	52.1
trnN-GUU-ycf1_p4	CCCCAAGTAGGATTCGAACC	TTACCTGGTATAAACGGCAT	55.0	40.0	57.7	54.1	528-529 bp	1.0	52.1
trnN-GUU-ycf1_p5	AGGATTCGAACCTACGACCA	ATTACCTGGTATAAACGGCAT	50.0	38.1	58.2	54.8	521-522 bp	1.0	50.7