Hotspot

trnI-CAU-ycf2

IRb · 86087-86148 · 62 bp

Back to Rubroshorea

Pi 0.179

MarkerSeek score 85.90

Primer available yes

Label trnI-CAU-ycf2

Feature Metadata

label_name: trnI-CAU-ycf2
feature_type: igs
region: IRb
length_bp: 62
variable_sites: 10
pi: 0.179
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 85.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-ycf2_p1	GTTGGGCGCTTTAACCATTC	TGAATTGACTTGGGTCCACG	50.0	50.0	58.0	58.1	196-216 bp	1.0	85.3
trnI-CAU-ycf2_p2	GTTGGGCGCTTTAACCATTC	GAATTGACTTGGGTCCACGA	50.0	50.0	58.0	57.8	195-215 bp	1.0	84.7
trnI-CAU-ycf2_p3	GTTGGGCGCTTTAACCATTC	AATTGACTTGGGTCCACGAG	50.0	50.0	58.0	57.8	194-214 bp	1.0	84.7
trnI-CAU-ycf2_p4	GTTGGGCGCTTTAACCATTC	TTGACTTGGGTCCACGAGTA	50.0	50.0	58.0	58.3	192-212 bp	1.0	83.9
trnI-CAU-ycf2_p5	GTTGGGCGCTTTAACCATTC	CCCACTGAATTGACTTGGGT	50.0	50.0	58.0	57.7	201-221 bp	1.0	83.7