Hotspot

psbE-petL

LSC · 65542-66643 · 1102 bp

Back to Rubroshorea

Pi 0.0306

MarkerSeek score 68.30

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1102
variable_sites: 33
pi: 0.0306
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	TGAGGTTATAGTTAAAGCCGCT	50.0	40.9	57.8	57.3	1181-1186 bp	1.0	60.6
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	TGAGGTTATAGTTAAAGCCGCT	55.0	40.9	58.2	57.3	1176-1181 bp	1.0	60.3
psbE-petL_p3	ACGAATAACCAACCCGCAAT	TGAGGTTATAGTTAAAGCCGCT	45.0	40.9	58.2	57.3	1267-1272 bp	1.0	60.2
psbE-petL_p4	TGCTGACGAATAACCAACCC	TGAGGTTATAGTTAAAGCCGCT	50.0	40.9	58.2	57.3	1272-1277 bp	1.0	60.1
psbE-petL_p5	CGAATAACCAACCCGCAATG	TGAGGTTATAGTTAAAGCCGCT	50.0	40.9	57.8	57.3	1266-1271 bp	1.0	60.1