Hotspot

trnT-GGU-psbD

LSC · 31392-32937 · 1546 bp

Back to Rubroshorea

Pi 0.0288

MarkerSeek score 67.80

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1546
variable_sites: 44
pi: 0.0288
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GAAACGGTCCCTACGTAACC	55.0	55.0	58.1	58.0	1688-1704 bp	1.0	82.4
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GAAACGGTCCCTACGTAACC	50.0	55.0	58.1	58.0	1691-1707 bp	1.0	82.2
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	GAAACGGTCCCTACGTAACC	50.0	55.0	58.3	58.0	1665-1681 bp	1.0	80.7
trnT-GGU-psbD_p4	TGGTAGAGTAACGCCATGGT	GAAACGGTCCCTACGTAACC	50.0	55.0	58.4	58.0	1687-1703 bp	1.0	79.5
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	CGAAACGGTCCCTACGTAAC	55.0	55.0	58.1	58.4	1689-1705 bp	1.0	79.4