Hotspot

rps16-trnQ-UUG

LSC · 6137-7707 · 1571 bp

Back to Rubroshorea

Pi 0.1

MarkerSeek score 72.40

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1571
variable_sites: 89
pi: 0.1
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.57
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	TTTGGTCCCGGTGATCAAAG	45.0	50.0	58.1	58.1	1236-1661 bp	1.0	87.0
rps16-trnQ-UUG_p2	TCATGTCCTTCAAGTCGCAC	TTTGGTCCCGGTGATCAAAG	50.0	50.0	58.2	58.1	1247-1672 bp	1.0	86.5
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	CCGGTGATCAAAGGTTCGAA	45.0	50.0	58.1	58.2	1229-1654 bp	1.0	86.3
rps16-trnQ-UUG_p4	TCATGTCCTTCAAGTCGCAC	CCGGTGATCAAAGGTTCGAA	50.0	50.0	58.2	58.2	1240-1665 bp	1.0	85.7
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	AATAGACAGAATGGGGCGTG	45.0	50.0	58.1	57.7	1278-1703 bp	1.0	85.0