Hotspot

trnH-GUG-psbA

LSC · 146-333 · 188 bp

Back to Rubroshorea

Pi 0.0372

MarkerSeek score 73.90

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 188
variable_sites: 7
pi: 0.0372
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	55.0	55.0	58.1	57.9	258-275 bp	1.0	80.5
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	272-289 bp	1.0	80.3
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	271-288 bp	1.0	80.3
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	273-290 bp	1.0	80.3
trnH-GUG-psbA_p5	GATCCACAATCCACTGCCTT	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	278-295 bp	1.0	80.3