Hotspot

ycf3

LSC · 43362-45599 · 2238 bp

Pi 0.0051

MarkerSeek score 49.50

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 2238
variable_sites: 30
pi: 0.0051
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	AAAACTAAAAGGCGTCGGCT	TCTTTTCTTGCGCAGGATCT	45.0	45.0	58.4	57.5	2198-2357 bp	1.0	78.0
ycf3_p2	AAAACTAAAAGGCGTCGGCT	TGCGCAGGATCTTTTTCTTG	45.0	45.0	58.4	57.0	2190-2349 bp	1.0	73.9
ycf3_p3	AAAACTAAAAGGCGTCGGCT	GCGCAGGATCTTTTTCTTGT	45.0	45.0	58.4	57.0	2189-2348 bp	1.0	73.9
ycf3_p4	AAAACTAAAAGGCGTCGGCT	ACAATTCTTTTCTTGCGCAGG	45.0	42.9	58.4	58.0	2203-2362 bp	1.0	73.7
ycf3_p5	AAAACTAAAAGGCGTCGGCT	TCTTGCGCAGGATCTTTTTCT	45.0	42.9	58.4	58.2	2193-2352 bp	1.0	72.1