Hotspot

atpA

LSC · 10907-12424 · 1518 bp

Pi 0.00257

MarkerSeek score 52.10

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: LSC
length_bp: 1518
variable_sites: 13
pi: 0.00257
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	AGCCTTCCAAGCTAACGATG	TCAAGTTCGACAACGGGTTT	50.0	45.0	58.0	58.0	2173-2226 bp	1.0	82.4
atpA_p2	AGTTTCGGGTTCGGTTTTGA	TCAAGTTCGACAACGGGTTT	45.0	45.0	58.0	58.0	2238-2291 bp	1.0	82.2
atpA_p3	CCTAGCCTTCCAAGCTAACG	TCAAGTTCGACAACGGGTTT	55.0	45.0	58.1	58.0	2176-2229 bp	1.0	82.2
atpA_p4	AGCCTTCCAAGCTAACGATG	GGGAAGGGGCTATTGAACAG	50.0	55.0	58.0	57.9	2376-2428 bp	0.857143	75.8
atpA_p5	AGTTTCGGGTTCGGTTTTGA	GGGAAGGGGCTATTGAACAG	45.0	55.0	58.0	57.9	2441-2493 bp	0.857143	75.6