Hotspot

rpl32-trnL-UAG

SSC · 114438-115100 · 663 bp

Back to Rubia

Pi 0.0147
MarkerSeek score 60.60
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
663
variable_sites
26
pi
0.0147
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.98
markerseek_score
60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TGGACAGCGTTAAAAGCTTT TCCTAAGAGCAGCGTGTCTA 40.0 50.0 56.2 57.9 748-789 bp 1.0 71.1
rpl32-trnL-UAG_p2 TTGGACAGCGTTAAAAGCTT TCCTAAGAGCAGCGTGTCTA 40.0 50.0 56.2 57.9 749-790 bp 1.0 71.1
rpl32-trnL-UAG_p3 TGGACAGCGTTAAAAGCTTT AGCAGCGTGTCTACCAATTT 40.0 45.0 56.2 57.8 741-782 bp 1.0 70.6
rpl32-trnL-UAG_p4 TTGGACAGCGTTAAAAGCTT AGCAGCGTGTCTACCAATTT 40.0 45.0 56.2 57.8 742-783 bp 1.0 70.6
rpl32-trnL-UAG_p5 TGGACAGCGTTAAAAGCTTT CTAAGAGCAGCGTGTCTACC 40.0 55.0 56.2 57.8 746-787 bp 1.0 70.5