Hotspot

ndhF-rpl32

SSC · 113528-114275 · 748 bp

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Pi 0.0107
MarkerSeek score 54.10
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
748
variable_sites
27
pi
0.0107
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 AAGGGGAAATTGTGCCGATT GGGTTTTGTGGTTTTCGTGG 45.0 50.0 58.1 58.1 2004-2162 bp 1.0 85.2
ndhF-rpl32_p2 AGGGGAAATTGTGCCGATTT GGGTTTTGTGGTTTTCGTGG 45.0 50.0 58.1 58.1 2003-2161 bp 1.0 85.2
ndhF-rpl32_p3 GCAAGAGGTCGTGTAAACCA GGGTTTTGTGGTTTTCGTGG 50.0 50.0 58.1 58.1 1778-1936 bp 1.0 84.9
ndhF-rpl32_p4 TGCAAGAGGTCGTGTAAACC GGGTTTTGTGGTTTTCGTGG 50.0 50.0 58.1 58.1 1779-1937 bp 1.0 84.9
ndhF-rpl32_p5 AGGGGAAATTGTGCCGATTT AAGAACGAGTTGAGGGTTCG 45.0 50.0 58.1 57.8 2036-2194 bp 1.0 84.5