Hotspot

psbH-petB

LSC · 75538-76016 · 479 bp

Back to Rubia

Pi 0.00338
MarkerSeek score 57.90
Primer available yes
Label psbH-petB

Feature Metadata

label_name
psbH-petB
feature_type
igs
region
LSC
length_bp
479
variable_sites
5
pi
0.00338
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbH-petB_p1 CTCCTTTAATGGGTGTCGCA TGTTGATCCTTACCAGGCCT 50.0 50.0 57.9 58.3 693-698 bp 0.857143 71.5
psbH-petB_p2 ACTCCTTTAATGGGTGTCGC TGTTGATCCTTACCAGGCCT 50.0 50.0 57.9 58.3 694-699 bp 0.857143 71.5
psbH-petB_p3 GGTGTCGCAATGGCTCTATT TGTTGATCCTTACCAGGCCT 50.0 50.0 58.3 58.3 682-687 bp 0.857143 69.7
psbH-petB_p4 CTCCTTTAATGGGTGTCGCA ATGTTGATCCTTACCAGGCC 50.0 50.0 57.9 57.3 694-699 bp 0.857143 68.2
psbH-petB_p5 ACTCCTTTAATGGGTGTCGC ATGTTGATCCTTACCAGGCC 50.0 50.0 57.9 57.3 695-700 bp 0.857143 68.2