Hotspot

trnG-GCC-trnM-CAU

LSC · 37213-37404 · 192 bp

Back to Rubia

Pi 0.0155
MarkerSeek score 52.30
Primer available yes
Label trnG-GCC-trnM-CAU

Feature Metadata

label_name
trnG-GCC-trnM-CAU
feature_type
igs
region
LSC
length_bp
192
variable_sites
10
pi
0.0155
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnM-CAU_p1 TCTTTGCCAAGGAGAAGACG CTTGAGGTCACGGGTTCAAA 50.0 50.0 57.8 58.0 243-270 bp 1.0 85.4
trnG-GCC-trnM-CAU_p2 TCTTTGCCAAGGAGAAGACG GGGTAGAGCAGTTTGGTAGC 50.0 55.0 57.8 58.0 279-306 bp 1.0 85.1
trnG-GCC-trnM-CAU_p3 TCTTTGCCAAGGAGAAGACG TGAGGTCACGGGTTCAAATC 50.0 50.0 57.8 57.8 241-268 bp 1.0 84.3
trnG-GCC-trnM-CAU_p4 TCTTTGCCAAGGAGAAGACG TAGCTCGCAAGGCTCATAAC 50.0 50.0 57.8 57.8 263-290 bp 1.0 83.7
trnG-GCC-trnM-CAU_p5 TCTTTGCCAAGGAGAAGACG GTAGCTCGCAAGGCTCATAA 50.0 50.0 57.8 57.8 264-291 bp 1.0 83.6