Hotspot

rpoB

Genome · 59939-63127 · 3189 bp

Back to Roya

Pi 0.000627
MarkerSeek score 43.10
Primer available yes
Label rpoB

Feature Metadata

label_name
rpoB
feature_type
gene
region
Genome
length_bp
3189
variable_sites
2
pi
0.000627
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
43.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB_p1 CCGATTTGTACGTCCCAGTT TCCAAACAAAGAATAGTACCAGT 50.0 34.8 57.9 55.5 217 bp 1.0 40.4
rpoB_p2 ACATATGCCGCCGATTTGTA TCCAAACAAAGAATAGTACCAGT 45.0 34.8 57.8 55.5 227 bp 1.0 40.4
rpoB_p3 TACATATGCCGCCGATTTGT TCCAAACAAAGAATAGTACCAGT 45.0 34.8 57.8 55.5 228 bp 1.0 40.4
rpoB_p4 TCTACTACATATGCCGCCGA TCCAAACAAAGAATAGTACCAGT 50.0 34.8 58.1 55.5 233 bp 1.0 40.3
rpoB_p5 ACTACATATGCCGCCGATTT TCCAAACAAAGAATAGTACCAGT 45.0 34.8 57.5 55.5 230 bp 1.0 40.3