Hotspot

petD

Genome · 42463-43634 · 1172 bp

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Pi 0
MarkerSeek score 29.50
Primer available yes
Label petD

Feature Metadata

label_name
petD
feature_type
gene
region
Genome
length_bp
1172
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
29.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petD_p1 TTGTTATGAGAGATTAAATGTTGT CGAAGCCCACATGCTAAGAA 25.0 50.0 52.7 58.3 1329 bp 1.0 40.0
petD_p2 TTGTTATGAGAGATTAAATGTTGT TGAAAAGACGAAGCCCACAT 25.0 45.0 52.7 57.7 1337 bp 1.0 40.0
petD_p3 TTGTTATGAGAGATTAAATGTTGT GAAAAGACGAAGCCCACATG 25.0 50.0 52.7 57.4 1336 bp 1.0 40.0
petD_p4 TTGTTATGAGAGATTAAATGTTGT GACGAAGCCCACATGCTAAG 25.0 55.0 52.7 59.0 1331 bp 1.0 40.0
petD_p5 TTGTTATGAGAGATTAAATGTTGT AGCCCACATGCTAAGAATCA 25.0 45.0 52.7 56.9 1326 bp 1.0 40.0