Hotspot

ndhK

Genome · 87236-88057 · 822 bp

Back to Roya

Pi 0.00122
MarkerSeek score 59.40
Primer available yes
Label ndhK

Feature Metadata

label_name
ndhK
feature_type
gene
region
Genome
length_bp
822
variable_sites
1
pi
0.00122
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhK_p1 AATCGTCCTTGCATTTGGGA AGGTGTACTCGCATTTGTCG 45.0 50.0 57.8 58.3 957 bp 1.0 76.0
ndhK_p2 ATCGTCCTTGCATTTGGGAA AGGTGTACTCGCATTTGTCG 45.0 50.0 57.8 58.3 956 bp 1.0 76.0
ndhK_p3 AATCGTCCTTGCATTTGGGA GGTGTACTCGCATTTGTCGA 45.0 50.0 57.8 58.3 956 bp 1.0 76.0
ndhK_p4 ATCGTCCTTGCATTTGGGAA GGTGTACTCGCATTTGTCGA 45.0 50.0 57.8 58.3 955 bp 1.0 76.0
ndhK_p5 ATCGTCCTTGCATTTGGGAA TTTATGCATGGCGTAAGGGG 45.0 50.0 57.8 58.3 897 bp 1.0 75.9