Hotspot

ndhH

Genome · 114134-115321 · 1188 bp

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Pi 0
MarkerSeek score 27.20
Primer available yes
Label ndhH

Feature Metadata

label_name
ndhH
feature_type
gene
region
Genome
length_bp
1188
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
27.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhH_p1 TCTTCCTTCGAGGGTCATAGT AGTCATTAACAAGAGGAGAGA 47.6 38.1 57.6 53.3 1247 bp 1.0 40.0
ndhH_p2 TCTTCCTTCGAGGGTCATAGT AAGTCATTAACAAGAGGAGAGA 47.6 36.4 57.6 54.2 1248 bp 1.0 40.0
ndhH_p3 TCTTCCTTCGAGGGTCATAGT AAAGTCATTAACAAGAGGAGAGA 47.6 34.8 57.6 55.0 1249 bp 1.0 40.0
ndhH_p4 TCTTCCTTCGAGGGTCATAGT AGTCATTAACAAGAGGAGAGAT 47.6 36.4 57.6 54.0 1247 bp 1.0 40.0
ndhH_p5 AAATCTTCCTTCGAGGGTCA AGTCATTAACAAGAGGAGAGA 45.0 38.1 56.2 53.3 1250 bp 1.0 40.0