Hotspot

ndhA

Genome · 113035-114132 · 1098 bp

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Pi 0.000911
MarkerSeek score 59.30
Primer available yes
Label ndhA

Feature Metadata

label_name
ndhA
feature_type
gene
region
Genome
length_bp
1098
variable_sites
1
pi
0.000911
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhA_p1 ACACTTCATTGATTCAGGTGT TGACATTATCATGGGGGAGGT 38.1 47.6 55.4 58.2 1183 bp 1.0 41.7
ndhA_p2 ACACTTCATTGATTCAGGTGT ATCATGGGGGAGGTTGATCG 38.1 55.0 55.4 59.2 1176 bp 1.0 41.5
ndhA_p3 ACACTTCATTGATTCAGGTGT GGGGGAGGTTGATCGCTAAA 38.1 55.0 55.4 59.5 1171 bp 1.0 40.1
ndhA_p4 ACACTTCATTGATTCAGGTGT ACATTATCATGGGGGAGGTTG 38.1 47.6 55.4 57.4 1181 bp 1.0 40.1
ndhA_p5 ACACTTCATTGATTCAGGTGT TGACATTATCATGGGGGAGG 38.1 50.0 55.4 56.3 1183 bp 1.0 40.1