Hotspot

ccsA

Genome · 124362-125327 · 966 bp

Back to Roya

Pi 0.00104
MarkerSeek score 59.30
Primer available yes
Label ccsA

Feature Metadata

label_name
ccsA
feature_type
gene
region
Genome
length_bp
966
variable_sites
1
pi
0.00104
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA_p1 TGTTTTCTCAATAGATTGTCAGT ATCATCAAACACCACATGAT 30.4 35.0 54.0 52.8 1086 bp 1.0 40.1
ccsA_p2 AGAAATGAAGAAATAGCAAGAATGT ATCATCAAACACCACATGAT 28.0 35.0 55.5 52.8 1165 bp 1.0 40.1
ccsA_p3 ATGTTTTCTCAATAGATTGTCAGT ATCATCAAACACCACATGAT 29.2 35.0 54.5 52.8 1087 bp 1.0 40.1
ccsA_p4 TGAAGAAATAGCAAGAATGTATGA ATCATCAAACACCACATGAT 29.2 35.0 54.4 52.8 1160 bp 1.0 40.1
ccsA_p5 TGTTTTCTCAATAGATTGTCAGT TCATCAAACACCACATGATA 30.4 35.0 54.0 52.2 1085 bp 1.0 40.1