Hotspot

atpB

Genome · 89544-91025 · 1482 bp

Back to Roya

Pi 0.000675
MarkerSeek score 59.10
Primer available yes
Label atpB

Feature Metadata

label_name
atpB
feature_type
gene
region
Genome
length_bp
1482
variable_sites
1
pi
0.000675
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB_p1 GGTTAGGGGCCATTACACAA ACCAATTTGCCATAAGAGCA 50.0 40.0 57.5 55.6 1555 bp 1.0 56.6
atpB_p2 TCGGTTAGGGGCCATTACA ACCAATTTGCCATAAGAGCA 52.6 40.0 58.0 55.6 1557 bp 1.0 52.7
atpB_p3 CACCAATTTGACCGCTATTCG ACCAATTTGCCATAAGAGCA 47.6 40.0 57.9 55.6 1618 bp 1.0 52.3
atpB_p4 CGGTTAGGGGCCATTACAC ACCAATTTGCCATAAGAGCA 57.9 40.0 57.9 55.6 1556 bp 1.0 52.0
atpB_p5 GAGCGTGGTTTGGTAAAACA ACCAATTTGCCATAAGAGCA 45.0 40.0 56.9 55.6 1636 bp 1.0 51.6