Hotspot

ycf3

LSC · 44021-46035 · 2015 bp

Back to Rottboellia

Pi 0.0163
MarkerSeek score 59.70
Primer available yes
Label ycf3

Feature Metadata

label_name
ycf3
feature_type
gene
region
LSC
length_bp
2015
variable_sites
32
pi
0.0163
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3_p1 TCTTCAACCAAACCACCCTT TCTATATAGAATGGGTTGGGA 45.0 38.1 57.2 52.5 2047-2081 bp 1.0 43.1
ycf3_p2 TCTTCAACCAAACCACCCTT TCTATATAGAATGGGTTGGGAA 45.0 36.4 57.2 53.4 2047-2081 bp 1.0 43.1
ycf3_p3 TCTTCAACCAAACCACCCTT TTCTATATAGAATGGGTTGGGA 45.0 36.4 57.2 53.4 2048-2082 bp 1.0 43.1
ycf3_p4 TCTTCAACCAAACCACCCTT TTCTATATAGAATGGGTTGGGAA 45.0 34.8 57.2 54.3 2048-2082 bp 1.0 43.1
ycf3_p5 ATCTTCAACCAAACCACCCT TCTATATAGAATGGGTTGGGA 45.0 38.1 56.9 52.5 2048-2082 bp 1.0 43.1