Hotspot

psbL-psbF

LSC · 63610-63631 · 22 bp

Back to Rottboellia

Pi 0.0909

MarkerSeek score 73.80

Primer available yes

Label psbL-psbF

Feature Metadata

label_name: psbL-psbF
feature_type: igs
region: LSC
length_bp: 22
variable_sites: 2
pi: 0.0909
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbL-psbF_p1	CATTCGGGTTTGATTGTGTCA	CACGGATTAGCTGTACCCAC	42.9	55.0	57.1	58.1	113 bp	1.0	65.6
psbL-psbF_p2	CATTCGGGTTTGATTGTGTCA	TCACGGATTAGCTGTACCCA	42.9	50.0	57.1	58.1	114 bp	1.0	64.9
psbL-psbF_p3	CATTCGGGTTTGATTGTGTCA	GCTGTTCACGGATTAGCTGT	42.9	50.0	57.1	58.3	119 bp	1.0	63.9
psbL-psbF_p4	TCATTCGGGTTTGATTGTGTC	CACGGATTAGCTGTACCCAC	42.9	55.0	56.8	58.1	114 bp	1.0	63.4
psbL-psbF_p5	TGTTCATTCGGGTTTGATTGTG	CACGGATTAGCTGTACCCAC	40.9	55.0	57.8	58.1	117 bp	1.0	63.0