Hotspot

trnV-UAC-trnM-CAU

LSC · 53633-53862 · 230 bp

Back to Rottboellia

Pi 0.0227

MarkerSeek score 69.70

Primer available yes

Label trnV-UAC-trnM-CAU

Feature Metadata

label_name: trnV-UAC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 230
variable_sites: 5
pi: 0.0227
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-UAC-trnM-CAU_p1	AACGAGTTGCTCTACCGAAC	CCCGCCGTATGAAAGCAATA	50.0	50.0	57.9	58.4	308-314 bp	1.0	80.1
trnV-UAC-trnM-CAU_p2	AACGAGTTGCTCTACCGAAC	CCGCCGTATGAAAGCAATAC	50.0	50.0	57.9	57.3	307-313 bp	1.0	78.1
trnV-UAC-trnM-CAU_p3	CGAGTTGCTCTACCGAACTG	CCCGCCGTATGAAAGCAATA	55.0	50.0	58.4	58.4	306-312 bp	1.0	77.5
trnV-UAC-trnM-CAU_p4	AACGAGTTGCTCTACCGAAC	TTTGAACCAATGACTCCCGC	50.0	50.0	57.9	58.8	323-329 bp	1.0	77.1
trnV-UAC-trnM-CAU_p5	AACGAGTTGCTCTACCGAAC	AGCGAAGTAAGTCCAATGCT	50.0	45.0	57.9	57.2	380-386 bp	1.0	76.5