Hotspot

trnG-UCC-trnM-CAU

LSC · 14312-16260 · 1949 bp

Back to Rottboellia

Pi 0.0228

MarkerSeek score 60.40

Primer available yes

Label trnG-UCC-trnM-CAU

Feature Metadata

label_name: trnG-UCC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 1949
variable_sites: 44
pi: 0.0228
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnM-CAU_p1	ACGAATCACACTTTTACCACT	ACCACTAAGTGAAAAGCCCT	38.1	45.0	55.5	56.7	2063-2076 bp	1.0	43.4
trnG-UCC-trnM-CAU_p2	ACGAATCACACTTTTACCACT	TGAACCGATGACTTATGCCT	38.1	45.0	55.5	56.6	2033-2046 bp	1.0	43.3
trnG-UCC-trnM-CAU_p3	ACGAATCACACTTTTACCACT	GACTTCTGTCTTACCAAGGCA	38.1	47.6	55.5	57.6	2091-2104 bp	1.0	43.0
trnG-UCC-trnM-CAU_p4	ACGAATCACACTTTTACCACT	TGACTTCTGTCTTACCAAGGC	38.1	47.6	55.5	57.6	2092-2105 bp	1.0	43.0
trnG-UCC-trnM-CAU_p5	ACGAATCACACTTTTACCACT	CCGATGACTTATGCCTTACCA	38.1	47.6	55.5	57.5	2029-2042 bp	1.0	42.6