Hotspot

ycf1

SSC · 107346-112922 · 5577 bp

Back to Rotheca

Pi 0.364

MarkerSeek score 75.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5577
variable_sites: 1786
pi: 0.364
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CATTTATCTCCGACCGGGTG	GCGGGAGTTTGGTGGATTTA	55.0	50.0	58.1	58.2	588 bp	0.5	57.6
ycf1_p2	CATTTATCTCCGACCGGGTG	AGATCCTTTGCGGGAGTTTG	55.0	50.0	58.1	58.2	597 bp	0.5	57.5
ycf1_p3	CATTTATCTCCGACCGGGTG	GATCTTCCCACCACCTTGTG	55.0	55.0	58.1	58.2	868 bp	0.5	57.5
ycf1_p4	CATTTATCTCCGACCGGGTG	CCAGACAGACGAATGGGTTC	55.0	55.0	58.1	58.3	1223 bp	0.5	56.7
ycf1_p5	TCCACCCCTTTCTTAAGCCT	GCGGGAGTTTGGTGGATTTA	50.0	50.0	58.2	58.2	725 bp	0.5	56.7