Hotspot

psaC

SSC · 119201-119446 · 246 bp

Back to Rotheca

Pi 0.559

MarkerSeek score 74.00

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 114
pi: 0.559
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	GCGGGTTTGATGCGAATAAG	TGAGCACGGATTTTGTCGAT	50.0	45.0	57.9	57.9	508 bp	0.5	58.4
psaC_p2	GATCGCCCATTGATTCGTCT	TGAGCACGGATTTTGTCGAT	50.0	45.0	58.1	57.9	406 bp	0.5	58.2
psaC_p3	TCTGATCGCCCATTGATTCG	TGAGCACGGATTTTGTCGAT	50.0	45.0	58.1	57.9	409 bp	0.5	58.2
psaC_p4	GCGGGTTTGATGCGAATAAG	GAGAATCTCGAGTAACGGGC	50.0	55.0	57.9	57.9	899 bp	0.5	58.2
psaC_p5	GCGGGTTTGATGCGAATAAG	TTGAGCACGGATTTTGTCGA	50.0	45.0	57.9	58.1	509 bp	0.5	58.1