Hotspot

ndhH

SSC · 113706-114887 · 1182 bp

Back to Rotheca

Pi 0.495

MarkerSeek score 74.80

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 372
pi: 0.495
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.64
markerseek_score: 74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	GAAAACGTCAACGACTGCTG	CGTTGTTGTATCCCTGCAGA	50.0	50.0	58.0	57.9	1570 bp	0.5	59.2
ndhH_p2	TGAAGCGCGATACCATTTGA	CGTTGTTGTATCCCTGCAGA	45.0	50.0	58.0	57.9	1459 bp	0.5	59.1
ndhH_p3	GAAAACGTCAACGACTGCTG	AGCAGCCCTTAATCCACCTA	50.0	50.0	58.0	58.1	1883 bp	0.5	59.1
ndhH_p4	TTGGGAAAACGTCAACGACT	CGTTGTTGTATCCCTGCAGA	45.0	50.0	58.0	57.9	1574 bp	0.5	59.0
ndhH_p5	TGCATGGAAGAATGACTCGG	CGTTGTTGTATCCCTGCAGA	50.0	50.0	58.0	57.9	1394 bp	0.5	59.0