Hotspot

ndhG

SSC · 117914-118444 · 531 bp

Back to Rotheca

Pi 0.511

MarkerSeek score 79.30

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 230
pi: 0.511
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	TGCCGGCATCAATAATGGAA	CTTATTCGCATCAAACCCGC	45.0	50.0	57.9	57.9	1533 bp	0.5	58.6
ndhG_p2	TTGCCGGCATCAATAATGGA	CTTATTCGCATCAAACCCGC	45.0	50.0	57.9	57.9	1534 bp	0.5	58.6
ndhG_p3	ACATCAGAACGTTTCCGAGG	CTTATTCGCATCAAACCCGC	50.0	50.0	57.9	57.9	1822 bp	0.5	58.5
ndhG_p4	CATCAGAACGTTTCCGAGGT	CTTATTCGCATCAAACCCGC	50.0	50.0	57.9	57.9	1821 bp	0.5	58.5
ndhG_p5	TGCCGGCATCAATAATGGAA	AGACGAATCAATGGGCGATC	45.0	50.0	57.9	58.1	1635 bp	0.5	58.5