Hotspot

ndhF

SSC · 123447-125663 · 2217 bp

Back to Rotheca

Pi 0.45

MarkerSeek score 78.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2217
variable_sites: 806
pi: 0.45
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GTTTAGTTGTTTGTCGCGCA	TAGACCCGTTTCCGAAGAGT	45.0	50.0	57.9	58.1	2987 bp	0.5	58.6
ndhF_p2	CACCCCTTCCCTTTCCAAAA	TAGACCCGTTTCCGAAGAGT	50.0	50.0	57.9	58.1	2901 bp	0.5	58.6
ndhF_p3	AGGTTCGGTGAATCGGAAAC	ATACCGTCGCCCCTTTTTAC	50.0	50.0	58.2	58.0	2649 bp	0.5	58.2
ndhF_p4	CACCCCTTCCCTTTCCAAAA	AAGGCGGATCCGAACAAAAT	50.0	45.0	57.9	58.2	2999 bp	0.5	57.9
ndhF_p5	AGGTTCGGTGAATCGGAAAC	TTTAGGCAGAATACCGTCGC	50.0	50.0	58.2	58.1	2659 bp	0.5	57.9