Hotspot

ndhA

SSC · 114889-116974 · 2086 bp

Back to Rotheca

Pi 0.477

MarkerSeek score 78.50

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2086
variable_sites: 804
pi: 0.477
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	GCCCGGTTTTATCAACTTGC	AGCCACGATTGATTCTCACC	50.0	50.0	58.0	58.0	2905 bp	0.5	59.7
ndhA_p2	GCCCGGTTTTATCAACTTGC	TCCATTATTGATGCCGGCAA	50.0	45.0	58.0	57.9	2734 bp	0.5	59.4
ndhA_p3	GCCCGGTTTTATCAACTTGC	TTCCATTATTGATGCCGGCA	50.0	45.0	58.0	57.9	2735 bp	0.5	59.4
ndhA_p4	CAATGTTACGAGCTTCCGGA	TGGCAGCTCGTATTGTTTGT	50.0	45.0	58.0	58.1	2743 bp	0.5	59.1
ndhA_p5	CAGGCTCTGGAAGGAATTCC	TCCATTATTGATGCCGGCAA	55.0	45.0	58.0	57.9	2973 bp	0.5	59.1