Hotspot

ndhE-psaC

SSC · 118951-119200 · 250 bp

Back to Rotheca

Pi 0.462

MarkerSeek score 75.90

Primer available yes

Label ndhE-psaC

Feature Metadata

label_name: ndhE-psaC
feature_type: igs
region: SSC
length_bp: 250
variable_sites: 104
pi: 0.462
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-psaC_p1	ACGTCCTGGTATGGGATCAT	TACATCCGTCCCAGGGTATC	50.0	55.0	57.9	58.0	1039 bp	0.5	59.1
ndhE-psaC_p2	ACGTCCTGGTATGGGATCAT	TGAGCACGGATTTTGTCGAT	50.0	45.0	57.9	57.9	1244 bp	0.5	58.4
ndhE-psaC_p3	ACGTCCTGGTATGGGATCAT	GACCCATACTGCGAGTTGTT	50.0	50.0	57.9	57.9	1171 bp	0.5	58.4
ndhE-psaC_p4	ACGTCCTGGTATGGGATCAT	TCCGTCCCAGGGTATCATTT	50.0	50.0	57.9	58.1	1035 bp	0.5	58.3
ndhE-psaC_p5	ACGTCCTGGTATGGGATCAT	ATCCGTCCCAGGGTATCATT	50.0	50.0	57.9	57.9	1036 bp	0.5	58.2