Hotspot

rps15-ndhH

SSC · 113602-113705 · 104 bp

Back to Rotheca

Pi 0.516

MarkerSeek score 75.30

Primer available yes

Label rps15-ndhH

Feature Metadata

label_name: rps15-ndhH
feature_type: igs
region: SSC
length_bp: 104
variable_sites: 47
pi: 0.516
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ndhH_p1	GAAAACGTCAACGACTGCTG	TCCGGAAGCTCGTAACATTG	50.0	50.0	58.0	58.0	889 bp	0.5	59.9
rps15-ndhH_p2	TTGGGAAAACGTCAACGACT	TCCGGAAGCTCGTAACATTG	45.0	50.0	58.0	58.0	893 bp	0.5	59.7
rps15-ndhH_p3	GAAAACGTCAACGACTGCTG	CGTAACATTGGCCCCGATAA	50.0	50.0	58.0	58.0	879 bp	0.5	59.6
rps15-ndhH_p4	TTGGGAAAACGTCAACGACT	CGTAACATTGGCCCCGATAA	45.0	50.0	58.0	58.0	883 bp	0.5	59.4
rps15-ndhH_p5	GAAAACGTCAACGACTGCTG	ATCAGTCGAAGAACACCGTG	50.0	50.0	58.0	57.9	291 bp	0.5	59.4