Hotspot

petA-psbJ

LSC · 61995-62935 · 941 bp

Back to Rosularia

Pi 0.0899
MarkerSeek score 82.70
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
941
variable_sites
79
pi
0.0899
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 CAATTGTCCGAAATGAATTTCT GGATTGGGTTCATCCTTGTAG 31.8 47.6 53.7 56.0 957-986 bp 1.0 50.5
petA-psbJ_p2 CAATTGTCCGAAATGAATTTCT GGATTGGGTTCATCCTTGTA 31.8 45.0 53.7 54.7 957-986 bp 1.0 50.5
petA-psbJ_p3 CAATTGTCCGAAATGAATTTC GGATTGGGTTCATCCTTGTAG 33.3 47.6 52.1 56.0 957-986 bp 1.0 50.5
petA-psbJ_p4 CAATTGTCCGAAATGAATTTCTAG GGATTGGGTTCATCCTTGTAG 33.3 47.6 54.9 56.0 957-986 bp 1.0 50.5
petA-psbJ_p5 CAATTGTCCGAAATGAATTTC GGATTGGGTTCATCCTTGTA 33.3 45.0 52.1 54.7 957-986 bp 1.0 50.5